The efficient procedure of embryogenic callus formation from anther in Capsicum pubescens Ruiz & Pav

The peppers or ajies (Capsicum spp.) are one of Peru's main productive crops, which is why it among the top ten countries in production and exports. Among species cultivated Peru Capsicum pubescens Ruiz & Pav., commonly called rocoto, represents most critical local national gastronomy. rocoto ecotype "Selva" "de Monte" characterized by its large size used to prepare relleno arequipeño. This crop presents restriction obtain homozygous lines for being self-incompatible. Using biotechnology, pure can be obtained from anther culture, obtained. objective was induce embryogenic callus formation culture Pav. Ecotype Monte". In results, 74.1% induced anthers with a longitudinal ratio 1.04 mm; induction carried out using Murashige Skoog medium added 2.0 mg/L 2,4-D. Likewise, somatic embryos globular stage evidenced after 12 weeks medium.